Figure 6.

nNOS enzyme activity in NAcSh is necessary for mediating CSDS-induced depression. A, Scatter plot showing nNOS-specific activity. n = 4, n = 4, n = 5, n = 5, and n = 5, respectively. Mean measured value of nNOS KO mice was regarded as the background and subtracted from measured value of each other group. B, Experimental design for C-F. C-F, Scatter plots showing time spent in interaction zone (C) and total distance traveled (D) in SIT (n = 12 for each group), immobility time in TST (E, n = 11 and n = 10, respectively), and sucrose preference percentage in SPT (F, n = 11 and n = 12, respectively). One mouse escaped after SIT. Two mice in inactive nNOS-eGFP group climbed up in TST and were excluded. Data are mean ± SEM. ***p < 0.001. n.s., not significant.