Figure 2.

Verification of single-cell RT-PCR method. A, Example of single-cell RT-PCR for a D2-MSN (left) and a D1-MSN (right) after whole-cell recordings in adult rat nucleus accumbens. β-actin was used as a positive control. B, Serial dilution of RNA from striatal tissue showing the sensitivity of pDYN primers (149 bp). C, Serial dilution of RNA from striatal tissue showing the sensitivity of pENK primers (220 bp). D, A second round of amplification is sufficient to allow for proenkephalin detection in samples containing 1 pg/µl of RNA.