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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Biochem J. 2020 Jul 17;477(13):2421–2438. doi: 10.1042/BCJ20200036

Figure 1. Deficiency of Runx1 in chondrocytes and osteoblasts caused the dwarfism, decreased body weight, and decreased bone density in mutant mice.

Figure 1.

(A) PCR genotyping images of Runx1 f/f and Runx1f/fCol2α1-cre mice. f, Runx1 allele carrying loxP sites; +, WT Runx1 allele; Δ, Runx1 allele with loxP floxed sequence deleted. (B) Gross appearances of newborn Runx1f/fCol2α1-cre mice and littermates. (C) Measurement of mice body lengths from 12-week-old Runx1f/fCol2α1-cre and Runx1f/f mice. (D) Quantification of mouse body lengths from 4-weeks-old to 12-weeks-old. (E) Quantification of mouse body weights from 1-month-old to 6-months-old. (F) X-ray analysis long bones from 4-week, 8-week, and 6-month-old Runx1f/fCol2α1-cre mice and littermate controls (f/f), white arrows referred to cortical bone and trabecular bone density differences between wt and mutant mice. (G) Micro computed tomography (μCT) examination was performed to determine the bone formation in 4-week murine femurs. (H) Quantification data of bone volume/tissue volume (BV/TV), the trabecular bone numbers (Tb. N), trabecular bone thickness (Tb. Tn), and connectivity density (Conn. D) in G. All data are presented as mean ± SD, n=4, NS denotes not significant, *p<0.05, **p<0.01, ***p<0.001. f/f denotes Runx1f/f; f/f/Δ denotes Runx1f/fCol2α1-cre.