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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Biochem J. 2020 Jul 17;477(13):2421–2438. doi: 10.1042/BCJ20200036

Figure 4. Runx1 deficiency in chondrocytes and osteoblasts leads to the impaired osteoblast differentiation and down-regulation of Ihh, CyclinD1 in murine femurs.

Figure 4.

Immunofluorescence (IF) staining of osteoblast related genes in newborn murine femurs with anti-Osx (A), anti-OCN (B), and anti-OPN (C). (D) IF staining by using anti-Ihh, (E) anti-CylinD1, and (F) anti-PTH1R in postnatal 9 murine growth plates. (G) Quantification of IF staining positive cells and area in (D)-(F). All data are presented as mean ± SD, n=6, NS denotes not significant, **p<0.01, ***p<0.001.