Immunofluorescence (IF) staining of osteoblast related genes in newborn murine femurs with anti-Osx (A), anti-OCN (B), and anti-OPN (C). (D) IF staining by using anti-Ihh, (E) anti-CylinD1, and (F) anti-PTH1R in postnatal 9 murine growth plates. (G) Quantification of IF staining positive cells and area in (D)-(F). All data are presented as mean ± SD, n=6, NS denotes not significant, **p<0.01, ***p<0.001.