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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Biochem J. 2020 Jul 17;477(13):2421–2438. doi: 10.1042/BCJ20200036

Figure 7. Runx1 cell-autonomously regulated osteoblasts and adipocytes differentiation.

Figure 7.

(A, B) Oil-Red staining of Runx1f/f and Runx1f/fCol2α1-cre newborn mice calvarial cells induced by osteogenesis medium for (A) 14 and (B) 21 days. (C) Western blot was performed to examine the protein levels of osteoblasts makers and adipocytes markers in Runx1f/f and Runx1f/fCol2α1-cre newborn mice calvarial cells induced by osteogenesis medium for 14 days and 21 days. (D) Quantification of (C). (E) qPCR for osteoblast markers and adipocytes markers expression in Runx1f/f and Runx1f/fCol2α1-cre newborn mice calvarial cells induced by osteogenesis medium for 14 days. All data are presented as mean ± SD, n=4; *p<0.05, **p<0.01, ***p<0.001 ****p<0.0001.