Skip to main content
. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Biochem J. 2020 Jul 17;477(13):2421–2438. doi: 10.1042/BCJ20200036

Figure 9. Runx1 regulated Ihh expression by directly binding to its promoter.

Figure 9.

(A) Schematic display of Ihh promoter region (−3919 to +270): TSS, predicted Runx1-binding sites, and ChIP primers positions. (B) ChIP analysis of Runx1 binding to the Ihh promoter in ATDC5 cell line induced 7 days using primers as indicated on the x-axis. Results are presented as ChIP/Input. (C). Agarose gel image using ChIP qPCR products in (B). (D) Ihh promoter fragments were inserted into pGL3-basic vector. ATDC5 cells were co-transfected with pGL3-Ihh −84bp, −456bp, −1280bp and Runx1. Luciferase was detected at 48 hours post transfection and normalized to β-gal activity. All data are presented as mean ± SD, n=4, NS denotes not significant, ***p<0.001.