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. 2021 Mar 10;17(3):e1009375. doi: 10.1371/journal.ppat.1009375

Fig 2. Induction of mICAM-1 is associated with reduced caseinolytic and elastolytic activity in lasR mutant filtrates.

Fig 2

BEAS-2B cells were stimulated for 24h with 30 μL filtrates of (A) the Early and isogenic Early ΔlasR mutant (+/- heat inactivation). mICAM-1 levels (MFI) were measured by flow cytometry, with SCFM (media) serving as negative control (—dashed line). Protease activity in wild-type and lasR mutant filtrates was characterized for (B) total protease activity using the Hide-Remazol Brilliant Blue assay, (C) for caseinolytic activity using the clearance zone diameter on skim milk agar and (D) for elastolytic activity using the Elastin-Congo Red assay. Results for (A) are shown as mean ± SD of one representative experiment (from ≥ 2 independent experiments, each with biological triplicates). Results for (B) and (D) are shown as mean ± SEM, with pooled data (n ≥ 4 biological replicates from two independent experiments). Results for (C) are representative of ≥ 2 independent experiments in biological duplicates. *P < 0.05; **P < 0.01; ***P < 0.001.