Fig 4. Neutrophil adhesion is increased in AEC stimulated with lasR mutant filtrates.

BEAS-2B cells were pre-stimulated with 30 μL filtrates of Early, Early ΔlasR and Late strains, then co-incubated with calcein-stained primary neutrophils (green). 20 ng/mL TNF-α served as positive control and SCFM served as negative control (—dashed line). Adherent neutrophils were visualized by confocal microscopy (10X objective) in (A) and quantified in (B). Results are shown as mean ± SD from one representative experiment (from 2 independent experiments, each with 3 biological replicates). *P < 0.05; **P < 0.01; ***P < 0.001.