Fig 6. lasR mutant induces greater pulmonary neutrophilic inflammation than wild-type infection.

Mice were infected with Early, Early ΔlasR or PBS embedded agar beads and lungs were harvested at 2 days p.i. for lung histopathology and immune cell counts. (A) Representative images of H&E stained lung sections (10X objective), demonstrating airway cross-sections and surrounding peri-bronchial tissues. Lung homogenates were analyzed by flow cytometry for enumeration of (B) total neutrophils (CD45+, Ly6G high, CD11c-) and (C) the percentage of neutrophils among all live single cells. Results are shown as medians, with each dot representing one mouse. *P < 0.05; **P < 0.01.