Fig 4. Blocking HIV-1 reverse transcription partially reduces m6A-deficient HIV-1 induced IFN-I expression in U937 cells.
(A) A schematic diagram of the experimental approach. (B-C) PMA-differentiated U937 cells in the nevirapine (NVP) groups were pre-treated with NVP (5 μM) for 2 hr prior to 2 hr incubation with HIV-1NL4-3 (250 pg of p24) generated from vector control or FTO-overexpressing HEK293T cells. NVP (5 μM) was maintained in the medium throughout the infection and subsequent culture. At 16 hpi, mRNA levels of (B) HIV-1 gag and (C) IFN-α and IFN-β were quantified by RT-qPCR. Spliced GAPDH was used as a normalization control (B-C). The results are shown as means ± S.D. of triplicated samples. One representative experiment of four repeats is shown. Two-way ANOVA was used for statistical analysis. * P ≤ 0.05, **** P ≤ 0.0001.