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. 2021 Mar 10;17(3):e1009421. doi: 10.1371/journal.ppat.1009421

Fig 11. MDA5 has no specific role in m6A modification of HIV-1 RNA to induce IFN-I expression.

Fig 11

(A) MDA5 expression levels were measured by Western blotting using control (shCtrl) and stable MDA5 knockdown (shMDA5) U937 cells. (B) shCtrl and shMDA5 U937 cells were transfected with poly(I:C). At 16 hr post-transfection, cells were collected for the analysis of IFN-α and IFN-β mRNA levels by RT-qPCR. The results are shown as means ± S.D. of three repeats with similar results. * P < 0.05, ** P < 0.005. (C and D) PMA-differentiated shCtrl and shMDA5 U937 cells were transfected with 250 ng of RNA oligo 1 (C) or oligo 2 (D). At 16 h post-transfection, cells were collected for the analysis of IFN-α and IFN-β mRNA levels by RT-qPCR. * P < 0.05, ** P < 0.005. The results are shown as means ± S.D. of three repeated experiments.