FIG. 4.

Example of workflow. Specifically designed in vitro engineered models are adopted to mimic the in vivo environment and test disease-mimicking cells. The in vitro experiments inform the development of computational models of tissue mechanics and Notch signaling as perturbed by the genetic mutation under investigation. The computational model predictions can be validated with further in vitro experiments, completing the cycle of model information and validation. Ideally, this process should lead to increased model credibility and reliable in vivo predictions (the scheme of the microfluidic device is adapted from Ref. van Engeland et al.⁶¹).