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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: J Mol Biol. 2017 Nov 20;430(1):58–68. doi: 10.1016/j.jmb.2017.11.005

Fig. 4.

Fig. 4.

In vitro transcription assay results. All transcription assays were repeated 2–4 times with similar results. (a) Results of a typical in vitro transcription assay. The transcripts are identified in left margin. (b–d) Quantification of the representative in vitro transcription assays in panel A. The right y-axis represents the relative pR and pL transcription levels against no-protein control, whereas the left y-axis shows that of pRM. The x-axis is shown in log scale. CI titration (B), Cro titration (C), and in vitro transcription assay (D) with increasing amounts of Cro and a fixed amount of CI (120 nM).