Fig. 11.

CRT0066101 blocks bladder carcinoma cells at G2/M phase as analyzed by flow cytometry. UMUC1 and T24T cell lines were exposed to increasing concentrations (0, 0.5, 1, 2, 2.5, and 3 µM) of CRT0066101 for 3 days. Cells were then harvested, stained, and the propidium iodide-labeled cells were analyzed for their cell cycle status by flow cytometry, as described in “Materials and methods”. The percentage of UMUC1 cells in each phase of the cell cycle was calculated using FlowJo v14.0 and is indicated in each cell cycle profile (a). Each assay was performed twice and one profile for each treatment is shown in a. The average numeric data of the two biological replicates for both UMUC1 and T24T cells are presented in b. Note the accumulation of the cells in the G2/M phase in both UMUC1 and T24T cell lines following CRT0066101 treatment (a, b)