Fig. 4.

Immunoblot analysis of PKD1, PKD2, and PKD3 protein levels in human urothelial bladder carcinoma cell lines. Four bladder cancer cell lines (SCaBER, UMUC5, UMUC1, and 5637) were used for analysis of PKD1 protein expression, while 8 bladder tumor cell lines (TCCSUP, T24T, T24, UMUC3, SCaBER, UMUC5, UMUC1, and 5637) were utilized for analysis of PKD2 and PKD3 protein levels. Total cellular protein was extracted from these cell lines, and a total of 80 µg cell extract protein was subjected to SDS-PAGE and immunoblotted with an antibody against PKD1, PKD2, or PKD3, as described in Materials and Methods. β-Actin was used as the control to verify equal protein loading and transfer