Fig. 9.

Filtered backprojection and iterative slice reconstructions of fluorescence overlaid onto absorption of a porcine lymph node embedded with fluorescent-stained cancer cell spheroids. All images were scaled independently and thresholded for visualization, such that 90% of fluorescence signal above the background is shown. MLEM reconstructions were resampled to match that of the FBP reconstruction. Scale bars are 2 mm. MLEM: maximum-likelihood expectation-maximization, FBP: filtered backprojection