Figure 3.

TRDMT1^G155V expression diminishes m⁵C and the response of repair factors at damage sites. (A) TRDMT1 KO U2OS-TRE cells transfected with TA-KR and TRDMT1^WTor GFP-TRDMT1^G155Vafter pre-treated cells with or without siTRIM28, following with treatment described in Figure 2D. The fold increase of intensity of GFP-TRDMT1 ^WT or GFP-TRDMT1^G155V at sites of TA-KR was shown (n = 3, 10 cells per replicate, mean ± SD). (BandC) TRDMT1 KO U2OS cells were transfected with TA-KR and Myc-TRDMT1^WT/TRDMT1^G155V following with treatment with 2D and stained for m⁵C (B) and RAD51 or RAD52 (C). (D) Quantification of recruitment of RAD51 and RAD52 at TA-KR sites (n = 3, 50 cells per replicate, mean ± SD).