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. 2021 Mar 16;10:e62250. doi: 10.7554/eLife.62250

Figure 3. The proportion of EE cells and EE progenitors increases during aging.

Figure 3.

(A) Confocal images showing fluorescent immunohistochemistry on posterior midguts of young, mid-age, and old flies (ages indicated) showing the expression of GFP driven by the Pros promoter (green, marking EEs) and DAPI (blue). Quantification of the EEs as a percentage of total DAPI cells during aging is shown on the right. One-way ANOVA followed by Dunnett’s multiple comparisons test: ****, p<0.0001. Young: n = 10, N = 3; Mid: n = 9, N = 2; Old: n = 8, N = 3. (B) Staining of young and old posterior midguts for YFP (green, marking ISCs), Pros (red), and DAPI (blue). Arrowheads point to Pros+ EEs (red) or Pros/YFP+ EE progenitors (green). Quantification of the proportion of EEs as a percentage of total DAPI cells as well as the proportion of EE progenitor cells as a percentage of total YFP+ ISCs are shown on the right. Two-tailed t-test: ***, p=0.0005. Young: n = 9, N = 3; Old: n = 6, N = 3. n: number of posterior midguts analyzed; N: number of independent experiments performed with similar results and a similar n. Data represented as mean ± SD.