Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
UAS-mcherry-RNAi | Bloomington Drosophila Stock Center | BDSC:35785; RRID:BDSC 35785 |
|
| Genetic reagent (D. melanogaster) |
UAS-w-RNAi | Bloomington Drosophila Stock Center | BDSC:25785; RRID:BDSC 25785 |
|
| Genetic reagent (D. melanogaster) |
UAS-Pc-RNAi | Bloomington Drosophila Stock Center | BDSC:36070; RRID:BDSC 36070 |
|
| Genetic reagent (D. melanogaster) |
UAS-Pc-RNAi | Bloomington Drosophila Stock Center | BDSC:33622; RRID:BDSC 33622 |
|
| Genetic reagent (D. melanogaster) |
UAS-E(z)-RNAi | Bloomington Drosophila Stock Center | BDSC:33659; RRID:BDSC 33659 |
|
| Genetic reagent (D. melanogaster) |
UAS-E(z)-RNAi | Bloomington Drosophila Stock Center | BDSC:36068; RRID:BDSC 36068 |
|
| Genetic reagent (D. melanogaster) |
trxE2, FRT82B/Tm6C | Bloomington Drosophila Stock Center | BDSC:24160; RRID:BDSC 24160 |
|
| Genetic reagent (D. melanogaster) |
FRT82B | Bloomington Drosophila Stock Center | BDSC:2051; RRID:BDSC 2051 |
|
| Genetic reagent (D. melanogaster) |
Pc1/TM1 | Bloomington Drosophila Stock Center | BDSC:1728; RRID:BDSC 1728 |
|
| Genetic reagent (D. melanogaster) |
UAS-eGFP | Bloomington Drosophila Stock Center | BDSC:6874; RRID:BDSC 6874 |
|
| Genetic reagent (D. melanogaster) |
OregonR | Bloomington Drosophila Stock Center | BDSC:5; RRID:BDSC 5 |
|
| Genetic reagent (D. melanogaster) |
UAS-trx-RNAi | Vienna Drosophila Resource Center | Transformant ID: GD37715 | |
| Genetic reagent (D. melanogaster) |
UAS-puc-RNAi | Vienna Drosophila Resource Center | Transformant ID: GD3018 | |
| Genetic reagent (D. melanogaster) |
UAS-sc-3HA | Fly ORF | F000085 | |
| Genetic reagent (D. melanogaster) |
ProsV1-Gal4 | Grosjean et al., 2001 | N/A | J. Korzelius |
| Genetic reagent (D. melanogaster) |
WDah | L. Partridge | N/A | |
| Genetic reagent (D. melanogaster) |
esg-Gal4, UAS-GFP | S. Hayashi, RIKEN, Japan | N/A | |
| Genetic reagent (D. melanogaster) |
esg-Gal4, UAS-2xEYFP/Cyo; Su(H)GBE-G80/TM3 | Wang et al., 2014 | N/A | S. Hou, NIH, USA |
| Genetic reagent (D. melanogaster) |
esg-Gal4, UAS-2xEYFP/Cyo; Su(H)GBE-G80, tub-Gal80ts/TM3 (ISCts) | Wang et al., 2014 | N/A | S. Hou, NIH, USA |
| Genetic reagent (D. melanogaster) |
w; esg-Gal4, UAS-GFP, tubGal80ts/Cyo; UAS-Flp, act>>CD2>>G4/TM6B (esg-F/Ots) | B. A. Edgar | N/A | |
| Antibody | anti-Prospero (mouse monoclonal) | DSHB | Cat# MR1A; RRID:AB_528440 |
IF (1:250) |
| Antibody | anti-H3K27me2 (rabbit polyclonal) | Abcam | Cat# ab24684; RRID:AB_448222 |
IF (1:500) |
| Antibody | anti-GFP (chicken polyclonal) | Abcam | Cat# ab13970; RRID:AB_300798 |
IF (1:2000) |
| Antibody | anti-phospho-histone H3 (rabbit polyclonal) | Sigma-Aldrich | Cat# 06–570 RRID:AB_310177 |
IF (1:1000) |
| Other | Hoechst 33342 | Thermo Fisher Scientific | Cat# 62249 | IF (1:1000) |
| Commercial assay or kit | RNeasy Mikro Kit | Qiagen | Cat# 74004 | |
| Commercial assay or kit | Nextera DNA Sample prep kit | Illumina | Cat# FC-131–1024 |
|
| Commercial assay or kit | MiniElute Reaction Cleanup kit | Qiagen | Cat# 28204 | |
| Commercial assay or kit | NEBNext High-Fidelity 2X PCR Master Mix | New England Biolabs | Cat #M0541 | |
| Commercial assay or kit | Chromium Single Cell 3’ Library and Gel bead kit v2 | 10x Genomics | PN-120237 | |
| Software, algorithm | FIJI is just ImageJ (FIJI) | Schindelin et al., 2012 | RRID:SCR_002285 | https://fiji.sc/ |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | https://www.graphpad.com/scientific-software/prism/ |
| Software, algorithm | Partek Flow Genomic Analysis Software | Partek | https://www.partek.com/partek-flow/ | |
| Software, algorithm | Adobe Photoshop and Illustrator | Adobe | RRID:SCR_014199; RRID:SCR_010279 |
https://www.adobe.com/ |