Figure 4.

Compounds 1 and 2 weakly attenuated the EB infectivity. Chlamydia trachomatis L2 EBs were incubated with 2, 8, or 32 μM compound 1 (a) or 2 (b) at 37°C or 4°C for 1 or 4 h. After washes, EBs were used to infect HeLa cell. At 36 hpi, the inclusion was counted as described in “Materials and Methods.” Data are presented as mean ± SD of three independent assays. ∗∗p < 0.01, compared 4°C with 37°C by Student's t-tests. ^#p < 0.05, compared with each DMSO control sample by Student's t-tests.