Table 3.
Antimicrobial activity of T. polium subsp. polium and M. graeca essential oils using disc diffusion method.
| Inhibition zones diameter (mm)∗ | ||||||
|---|---|---|---|---|---|---|
| Essential oils | Antimicrobial agents | |||||
| T. polium subsp. polium | M. graeca | Gentamicin (15 μg) | Vancomycin (30 µg) | Streptomycin 25 µg | Amphotericin (10 μg) | |
| S. aureus CECT 976 | 9.66 ± 1.52+ | 22 ± 1+++ | 34.33 ± 0.57 | 30.66 ± 0.57 | nt | nt |
| B. subtilis DSM 6633 | 23 ± 2+++ | 28.33 ± 1.52+++ | 26 ± 1 | 27.66 ± 0.57 | nt | nt |
| L. innocua CECT 4030 | 11.33 ± 1.52+ | 19.33 ± 1.15++ | 17.66 ± 0.57 | 25.33 ± 0.57 | nt | nt |
| E. coli K12 | 10.33 ± 1.52+ | 17.66 ± 1.52++ | 20.33 ± 0.5 | 8 ± 00 | nt | nt |
| P. aeruginosa CECT 118 | 9.33 ± 0.57+ | 9.33 ± 1.52+ | 19 ± 1 | n.e | nt | nt |
| P. mirabilis | 21.33 ± 2.08+++ | 20 ± 2+++ | 28.66 ± 0.57 | 24.33 ± 0.57 | nt | nt |
| C. michiganensis 1616-3 | 52 ± 1+++ | >65 | nt | nt | 24.66 ± 0.57 | nt |
| P. savastanoi PSS2636-40) | 22 ± 1+++ | 49 ± 1+++ | nt | nt | 26.33 ± 0.57 | nt |
| C. albicans ATCC 10231 | 7.33 ± 0.57− | 44.33 ± 0.57+++ | nt | nt | nt | 18.66 ± 1.15 |
∗The diameter of the inhibition zones (mm), including diameter of disc (6 mm), is given as mean ± SD of triplicate experiments. nt: not tested; n.e: no effect. The sensitivity to the different oils was classified by the diameter of the inhibition halos as follows: not sensitive (−) for diameters less than 8 mm; sensitive (+) for diameters 9–14 mm; very sensitive (++) for diameters 15–19 mm; and extremely sensitive (+++) for diameters larger than 20 mm.