Skip to main content
. 2021 Mar 9;12:608890. doi: 10.3389/fimmu.2021.608890

Figure 1.

Figure 1

NCR expression on T cells in HTLV-1 infection. Cryopreserved PBMCs from HTLV-1-infected asymptomatic carriers (AC, n = 20), HTLV-1-infected individuals diagnosed with HAM/TSP (HAM/TSP, n = 6) and seronegative controls (SC, n = 12) were thawed and immunophenotyped for NCR expression on CD4 and CD8 T cells. Frequency of single expression of PD-1, TIGIT, TIM-3 and Lag-3 on CD4 T cells (A), CD8 T cells (B) are shown in the upper panels. Lower panels show frequency of multiple negative checkpoint receptor expression on CD4 T cells (C) and CD8 T cells (D). Double NCR expression includes PD-1 and TIGIT; triple NCR expression includes PD-1, TIGIT, TIM-3; quadruple NCR expression includes PD-1, TIGIT, TIM-3, LAG-3. Multiple NCR expression was determined using Boolean gating. Significance was calculated using Kruskal-Wallis multiple comparisons test (*p < 0.05; **p < 0.005; ***p < 0.0005).