FIGURE 1.

Scheme of the analysis of the properties of the PRGF and its effects on retinal cell cultures performed in this study. First, human and porcine blood was extracted and PRGF was obtained by plasma extraction. The effect of the PRGF was analyzed on three different types of cell cultures: pure RGCs or Müller cell cultures, and co-cultures of both these cell types. In addition, the presence of cytokines in the human and porcine PRGF was quantified through a Q-plex assay. The heat-inactivated form of PRGFs were also analyzed. After confirming the presence of cytokines in the PRGF, we checked the effect of the presence of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) on the RGCs cultures and co-cultures. We heat-inactivated the PRGF, and we added the inflammatory drug dexamethasone (Dex) or antibodies against cytokines (anti-cyt) to the PRGF in order to analyze its effect on the cell cultures. The survival effect of porcine and human PRGF on pure Müller cells cultures were also assessed.