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. 2021 Mar 9;12:606275. doi: 10.3389/fphar.2021.606275

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Copyright © 2021 Ruzafa, Pereiro, Fonollosa, Araiz, Acera and Vecino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of PRGF on RGC cultures. Images of control adult pig RGC cultures (A) or RGCs maintained in the presence of PRGF (B). The RGCs were labeled with antibodies against βIII-Tubulin (red) staining the nuclei with DAPI (blue). The percentage of RGCs (C) relative to the controls are represented in the histograms. RGCs were cultured in the presence of cytokines (IL-1β, IL-6, and TNFα), 10% human PRGF (PRGF), PRGF inactivated by heat, PRGF with dexamethasone (Dex) and PRGF with antibodies against cytokines (anti-cyt). Significant differences relative to the controls are shown:*p < 0.05. Scale bar = 100 μm.