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. 2021 Mar 9;12:606275. doi: 10.3389/fphar.2021.606275

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Copyright © 2021 Ruzafa, Pereiro, Fonollosa, Araiz, Acera and Vecino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of PRGF on RGCs and Müller cells in co-culture. Control co-cultures of adult pig RGCs and Müller cells (A) and those maintained in the presence of PRGF (B). The RGCs (arrows) were labeled with antibodies against βIII-Tubulin (red) and Müller cells (arrowheads) with antibodies against vimentin (green) and the nuclei were labeled with DAPI (blue). The number of RGCs (C) and Müller cells (D) in the co-cultures relative to the total number of cells in the control condition (100%) are represented in the histograms. RGCs and Müller cells were co-cultured with cytokines (cyt: IL-1β, IL-6 and TNFα), 10% human PRGF (PRGF), heat inactivated PRGF, PRGF with dexamethasone (Dex) and PRGF with antibodies against the cytokines (anti-cyt). Significant differences relative to the control conditions are shown: *p < 0.05. Scale bar = 100 μm.