Fig. 7.

Yeast one-hybrid assay and OsSAMS1, OsNAP and OsNYC3 expression analysis. a Yeast one-hybrid assay of OsNAC109. The empty negative control pLacZi was co-transformed with effectors; b Yeast one-hybrid assay of the NAC domain of OsNAC109; c Diagram of the effector and reporter constructs used in d–f; REN indicates Renilla luciferase gene, and LUC indicates firefly luciferase gene; d–f Dual luciferase reporter assay of OsSAMS1 (d), OsNAP (e), and OsNYC3 (f), means ± SD, n = 3; **P < 0.01 by Student’s t test; g–i Expression levels of OsSAMS1 (g), OsNAP (h), and OsNYC3 (i) in WT and yl3 at the seedling stage, means ± SD, n = 3, *P < 0.05, and **P < 0.01 by Student’s t test