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. 2021 Mar 22;11:6521. doi: 10.1038/s41598-021-85180-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Monitoring of cardiopulmonary vitals and motor testing. (a) The grey region indicates the period of sonication. Mean change in pulse across all mice showed injection-associated (red arrows) physiological dip in heart rate that spontaneously resolves. No cardiac pause or apnea was observed. (b) Mean respiratory rate recording showed no significant fluctuations or change. Shaded areas represent one standard deviation. (c) Weights test in FUS group revealed no difference in motor score before and after FUS (p = 0.8470). (d) After the second FUS, mice did show a significant trend with improved motor testing (p = 0.0301).