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. 2021 Mar 9;9:644667. doi: 10.3389/fcell.2021.644667

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Copyright © 2021 Ye, Zhang, Zhou, Tan, Xu, Yi, Yan, Xie, Zhang, Tian and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of AMPK during hiPSC-derived cardiomyocyte differentiation. (A) Western blot analysis of AMPK, phospho-AMPK, PGC-1α, CPT-1α, and α-actinin on days 0, 10, 20, and 30 during hiPSC-derived cardiomyocyte differentiation. RT-qPCR analysis of the genes regulating energy metabolism [PGC-1α (B) and CPT-1α (C)] and fatty acid transport and oxidation-related genes [FABP3 (D), FAT-CD36 (E), and SLC27a6 (F)] in hiPSC-derived cardiomyocytes on days 0, 10, 20, and 30 (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 one-way ANOVA.