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. 2021 Mar 22;11:6489. doi: 10.1038/s41598-021-85833-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protective effect of GF-EVs loaded with HSP70 from the etoposide-induced cytotoxicity. DLD1-scr (left) or DLD1-hsp70 (right) cells were preincubated in E-plates for 19–20 h before the addition of HSP70 at concentration of 2 μg/mL or GF-EVs loaded with HSP70 (10⁶ or 2 × 10⁶ particles per cell, appr. 1 μg/mL or 2 μg/mL of HSP70 respectively); 4 h later the etoposide (EP) was added to a final concentration of 20 μM in each well. Recording with the aid of xCELLigence equipment was started immediately after drug added and lasted for 60 h. In every experiment, each point in the plot represents an average recording of two wells. In each panel, data from one of two independent experiments are presented.