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. 2021 Mar 22;11:6556. doi: 10.1038/s41598-021-86120-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of chemoresistant MX-1 cell sublines. (A) Representative phase-contrast microscopic images demonstrating morphology of MX-1 cells and morphological changes in MX-1/D and MX-1/T cells cultured in media with doxorubicin and TPP⁺ respectively. Magnification—× 200. (B) Cytotoxicity of doxorubicin measurements with the MTT assay in the parental MX-1 cells and chemoresistant MX-1/D (cultured with 80 nM of doxorubicin), MX-1/T (cultured with ABCB1 transporter activator TPP⁺ (128 nM)) and MX-1/TD (MX-1/T cells cultured in media supplemented with up to 1280 nM of doxorubicin for a week). The indicated P-value was calculated at the end point of the experiment, compared the viability of each chemoresistant cell sublines with wild-type cells. Resistance index was calculated IC50 values of chemoresistant cells dividing by IC50 of parental MX-1 cells. (C) Heatmap showing the gene expression profile of the parental MX-1 and chemoresistant MX-1/D, MX-1/T and MX-1/TD cells. The results were obtained by Human Gene Expression (v2) 8 × 60 K microarrays (design ID 039494). (D) Venn diagram, showing the numbers of overlapping deregulated genes among MX-1/D, MX-1/T and MX-1/TD sublines compared to untreated MX-1 cells. Listed genes are common in the all investigated cells.