Fig. 5. Corticostriatal functional connectivity correlates with disrupted sociability in A350V mice.

a Seed-based comparison of the ACAv/d showing fcMRI statistical parametric maps (red-yellow) of positive correlations for WT (left) and A350V mice (right) (p < 0.05, corrected for multiple comparisons using whole-brain family-wise error (FWE) rate correction) and an fcMRI statistical parametric map (blue-light blue) of increased connectivity in A350V relative to WT showing significant increase in the dorsomedial CP (two-sample unpaired t test, p = 0.04, FWE cluster-corrected, with cluster-defining threshold of t(24) > 2.49, p < 0.01, voxel extent ≥ 5 and Bonferroni network-level corrected, with network-defining threshold of p < 0.0125). All maps are displayed on a downsampled version of the AMBC Atlas that matches the original fMRI resolution. Purple circles indicate the ACAd/v seed location. Black dashed circle indicates CP cluster that was found significant. b Seed-to-seed correlation between ACAd/v and CP showing increased connectivity in A350V mice. Colored circles indicate individual subject z(r) values. Coronal slices showing seed location for ACAd/v (purple) and CP (green) (top left). c Duration of time spent in close interaction with Stranger 1 (S1) and an object (O) in the three-chamber social preference test demonstrating that both A350V and WT prefer interacting with S1, although this preference is less pronounced in A350V. Colored circles indicate individual subject variability in sociability measurement with black circles indicating outlier in the data. A representative heat map analysis of A350V mouse is depicted (top center). d Connectivity between ACAd/v and CP in relation to sociability showing significant correlation for A350V mice only. Data indicate mean ± SEM. Mann–Whitney U-test (a); **p < 0.01. Two-way rmANOVA, with Holm–Bonferroni post hoc t test (c); *p < 0.05, **p < 0.01, ***p < 0.001. ACA anterior cingulate area, CP caudoputamen.