Figure 5.

Defective polarization to conditional medium of TSC1 cKO macrophages is mediated by mTOR-C/EBPβ in vitro. (A) Experimental design of the BMDMs from the WT and Lyz-TSC1 cKO mice, which were treated with CM from mouse renal TECs (mRTECs) cell line after hypoxia-reoxygenation (H/R) injury. (B) Representative dot plots of the CD206⁺ subset in the F4/80⁺ compartment in the WT or TSC1 cKO BMDMs treated with conditional medium. (C) Quantification of the proportion of the CD206⁺ subset in F4/80⁺ macrophages. (D) Relative expression of CD206 and Arginase-1 (Arg-1) in WT or TSC1 cKO BMDMs treated with CM. (E) Lyz-tsc1 cKO BMDMs were pretreated with Rapa (100 nM) for 1 h and then stimulated with CM for an additional 48 h. C/EBPβ expression in WT, Lyz-TSC1 cKO, and Lyz-TSC1 cKO treated with Rapa as determined by quantitative PCR after treatment with or without CM. In (C, D), the data are shown as the mean ± SD and were analyzed by an unpaired two-tailed Student’s t-test (n ≥ 3). *P < 0.05; **P < 0.01; NS, not significant.