Fig. 2. Selected Sumo targets differentially associated with proliferation and differentiation.

a Validation of the proteomic results of a selection of proteins by western blot with specific antibodies against the different proteins, after precipitation with Sumo antibodies or with control IgG and peptide elution (100%), under proliferation (0 days (d)) or differentiation conditions (4 d of RA treatment). Some proteins were validated with Sumo1 antibodies and others with Sumo2/3 antibodies. Inputs (1.5%) are also shown. Black arrowheads indicate unmodified proteins, while white arrowheads indicate sumoylated products. Asterisks denote nonspecific bands. b Proliferation (0 days) and differentiation conditions (4 days of RA treatment) were checked by analysis of the pluripotency marker Oct4 and the differentiation marker βIII-tubulin. α-Tubulin was determined as a loading marker. c The log₂ of the mean value of the ratio in experiment 1 and the inverse ratio in experiment 2 for selected proteins, either for Sumo1 (blue) or for Sumo2/3 (red), is represented.