Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 9;12:627020. doi: 10.3389/fimmu.2021.627020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Patten, Ferrer, Chen, Kolitz, Rai, Allen, Barrientos, Ioannou, Ramsay and Chiorazzi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Immunohistochemistry demonstrating the presence and localization of non-B and non-T cells of donor or recipient origin in engrafted spleens. (A) 20x (single color PAX5 and CD68) and 60x (dual color) IH images of representative spleens aggregated from 5 independent experiments; results with CLL1279 are demonstrated here. Aggregates contain CD68⁺ cells that are B lymphocytes as indicated by co-localization with PAX5 in dual color staining. Flow cytometry further demonstrates that CD5⁺CD19⁺ cells express CD68 upon transfer into NSG mice. (B) 20x and 40x magnification view of human CD20⁺ and CD3⁺ cells and mouse CD31⁺ cells by immunofluorescence of CLL-engrafted spleens; staining from a representative case of at least 10 independent experiments. (C) 20x and 40x magnification view of human CD20⁺ and CD3⁺ cells and mouse F4/80⁺ and CD21⁺/35⁺ cells after immunofluorescence staining of the same case.