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. 2021 Mar 9;12:635962. doi: 10.3389/fpls.2021.635962

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Copyright © 2021 Kaulfürst-Soboll, Mertens-Beer, Brehler, Albert and von Schaewen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GNTI- and MANII-RNAi plants show opposite growth phenotypes. Micro-Tom wild-type (wt) next to GNTI-RNAi (#20) and MANII-RNAi (#14) plants in the greenhouse. Around the age of 4 months, leaves of wt plants started to develop yellow rims (center), while premature senescence was visible for GNTI-RNAi plants, with yellow to necrotic leaf sectors (center left). At this stage, MANII-RNAi plants showed no signs of senescence, but often rolled-up leaf rims (center right). When cut back, GNTI-RNAi plants were able to grow new green sprouts (bottom left). Leaves and fruits of MANII-RNAi plants had a darker, matte appearance (center and bottom right). For details on plant growth and flower and fruit phenotypes, see Supplementary Figure S3.