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. 2021 Mar 9;12:635962. doi: 10.3389/fpls.2021.635962

FIGURE 5.

FIGURE 5

ManII-RNAi fruits resemble wild-type, except for seed number and mass. (A) Comparison of GNTI-RNAi (#20) and MANII-RNAi (#15) Micro-Tom plants in the greenhouse. Mature GNTI-RNAi fruits show yellow-green areas, whereas MANII-RNAi fruits develop normally. (B) Stalk-associated fruit parts of GNTI-RNAi (#20) remain incompletely ripe (green parts) and show signs of necrosis (arrows), whereas MANII-RNAi (#15) fruits look normal but contain less seeds. (C) Acid-washed dried and stored tomato seeds, shown for Micro-Tom wild-type (wt), and exemplarily for a GNTI-RNAi (GTI) and MANII-RNAi line (MII). Each box measures 5 mm × 5 mm. (D) MANII-RNAi seeds are heavier and GNTI-RNAi seeds are slightly lighter than Micro-Tom wild-type. The box plot was compiled with seeds from several seed batches (biological replicates) of wild-type (wt; N = 14), GNTI-RNAi lines (GTI #20, N = 8; #45, N = 4), and MANII-RNAi lines (MII #11, N = 13; #13, N = 2; #14, N = 23; #15, N = 11; #18, N = 9). For details (one-way ANOVA), see Supplementary Excel Sheet.