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. 2021 Mar 22;5:26. doi: 10.1038/s41698-021-00160-9

Fig. 3. CXCL12 in tumors are expressed by Pdgfr+ lineage stromal cells.

Fig. 3

a scheme for mT (RFP)/mG (GFP) lineage tracing of CXCL12 expression in the stroma with PDGFRa-Cre and PDGFRb-Cre drivers in mice. b Representative genitourinary tracts from Pdgfra-cre;mTmG, HiMyc;Pdgfra-cre;mTmG, and HiMyc;Pdgfra-cre;mTmG mice (C57BL/6 background) fed chow at 12 months of age showing tumors (arrows) in HiMyc+ mice. c PDGFRa-Cre;mTmG and PDGFRb-Cre;mTmG lineage tracing in tumors from HiMyc+ mice fed chow. Cells that have expressed Cre driven by a PDGFR promoter, are mG+ (as detected by anti-GFP IF) due to loxP-flanked mT excision (mT fluorescence in other cells is lost in paraffin sections). Yellow arrows indicate that mG+ stroma expresses CXCL12. Tumor epithelium is defined by dashed line. d Ventral prostate from HiMyc mice fed chow subjected to IF with indicated antibodies. Note CXCL12 deposits adjacent to and co-localizing with (yellow) stromal cells expressing PDGFRα and PDGFRβ. DNA is blue. Scale bar = 100 µm.