FIGURE 3.

Response of GmNHX5-silencing soybean plants to salt stress. (A) Silencing efficiency of GmNHX5 in TRV-inoculated plants. The relative expression of GmNHX5 was detected using RT-qPCR, and normalized to the values measured in TRV: 00-#1. Actin was used as a reference gene. Each value represents the mean ± SE of three technical replicates. Different letters above the bars indicate significant differences (P < 0.05 by one-way ANOVA and Duncan’s multiple comparison). (B) Phenotype of GmNHX5-silencing plants under salt stress condition. TRV-inoculated soybean plants were subjected to 0- or 200 mM NaCl solution treatment for 5 days. The images on the right show the phenotype of simple (top) and ternately compound leaves (bottom) of indicated plants under salt stress.