Skip to main content
. 2021 Mar 9;12:625957. doi: 10.3389/fimmu.2021.625957

Figure 1.

Figure 1

Hydrogen significantly alleviated LPS-induced acute lung injury. LPS (10 mg/kg i.p.) was used to establish endotoxemia mice. H2 (4% H2 inhalation) was used for treatment. The survival rate (A) of mice was measured every day for 7 consecutive days (n = 11 in each group). (B) Lung injury was significantly reduced at 12 and 24 h in the LPS + H2 group (n = 3 in each group). (C) Representative photographs of lung tissues after LPS injection at 6, 12, and 24 h (n = 3 in each group). (D) Hematoxylin- and eosin-stained sections of the lung tissues of mice at 6, 12, and 24 h. The “arrow” in the figure showed red blood cells, fibrin strands and hyaline membranes. Magnification: 400 × (n = 3 in each mice). Neutrophil proportion in blood (E) assessed by flow cytometric analysis and quantitative data of the neutrophil proportion (F) analyzed by flow cytometry at 12 h (n = 3 in each group). (G) IL-6 concentration in mouse plasma by ELISA at 6, 12, and 24 h (n = 3 in each group). A significant difference was revealed by one-way ANOVA (**P < 0.01, ***P < 0.001 vs. Control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS-treated group; Bonferroni post-hoc tests).