Figure 2.

Hydrogen reduced endotoxemia-related pulmonary edema and improved the pulmonary circulation. LPS (10 mg/kg i.p.) was used to establish endotoxemia mice. H₂ (4% H₂ inhalation) was used for treatment. (A) Representative images of B-mode ultrasound. The “arrow” in the figure showed A line and B line. The pulmonary edema degree was assessed by the percentage of area scanned by the B line at 6, 12, and 24 h (n = 4 in each group). (B) Ultrasonic Doppler assessment on heart rates and blood flow velocity in the subclavian artery of mice at 6, 12, and 24 h (n = 4 in each group). A significant difference was revealed following one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control; ^##P < 0.01, ^###P < 0.001 vs. LPS-treated group; Bonferroni post-hoc tests).