Figure 3.

Hydrogen significantly suppressed LPS-induced increase of tissue factor (TF) in lungs and serum from mice. LPS (10 mg/kg, i.p.) was used to establish endotoxemia mice. H₂ (4% H₂ inhalation) was used for treatment. Effect of hydrogen on the expression of TF in lungs (A) and serum (B) were measured by western blot analysis and the densitometry values were normalized to β-actin and transferrin at 6, 12, and 24 h (n = 4 in each group). (C) Tissue paraffin sections of lungs subjected to TF immunohistochemical staining at 6, 12, and 24 h (n = 4 in each group). (D) TF expression by immunofluorescence detection in the lung tissues of mice at 12 h (n = 3 in each group). Significant difference was revealed following one-way ANOVA (*P < 0.05, ***P < 0.001 vs. Control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. LPS-treated group; Bonferroni post-hoc tests).