Figure 5.

Hydrogen significantly upregulated the LPS-induced increase of Trx1 in lung and serum from mice. LPS (10 mg/kg, i.p.) was used to establish endotoxemia mice. H₂ (4% H₂ inhalation) was used for treatment. PX₁₂ (12 mg/Kg) was intraperitoneally injected in mice as an inhibitor of Trx1. Effect of hydrogen on the expression of Trx1 in lungs (A) and serum (B) were measured by western blot analysis and the densitometry values were normalized to β-actin at 6, 12, and 24 h (n = 4 of each group). (C) Tissue paraffin sections of lungs subjected to Trx1 immunohistochemical staining at 6, 12, and 24 h (n = 4 of each group). Effect of hydrogen and PX₁₂ on the expression of TF in lung (D) and serum (F) at 12 h were measured by western blot analysis and the densitometry values were normalized to β-actin (n = 4 in each group). Effect of hydrogen and PX12 on the expression of MMP-9 in lung (E) and serum (G) were measured by western blot analysis and the densitometry values were normalized at 12 h (n = 4 in each group). Significant differences were revealed following one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. LPS-treated group; ^$P < 0.05, ^$$$P < 0.001 vs. LPS + H2 group; Bonferroni post-hoc tests).