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. 2021 Mar 9;12:625957. doi: 10.3389/fimmu.2021.625957

Figure 6.

Figure 6

Hydrogen significantly suppressed the LPS-induced increase of tissue factor (TF) and matrix metalloproteinase (MMP)-2/9 in HUVEC-C and THP-1 cells. LPS (0.3 μg/ml) was used to establish endotoxemia model in HUVEC-C cells. LPS (1 μg/ml) was used to establish endotoxemia model in THP-1 cells. H2 (65% H2) was used to treat both HUVEC-C and THP-1 cells. TF (A,C) and MMP-9 (B,D) expression by immunofluorescence detection in HUVEC-C cells at 12 h (n = 3 in each group). Effect of hydrogen on the expression of TF in THP-1 (E) and HUVEC-C cells (F) were measured by western blot analysis and the densitometry values were normalized to β-actin at 6, 12, and 24 h (n = 3 in each group). Effect of hydrogen on the expression of MMP-2/9 in THP-1 (G) and HUVEC-C cells (H) were measured by gelatin zymography and the densitometry values were normalized at 6, 12, and 24 h (n = 3 in each group). Significant differences were revealed following one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LPS-treated group; Bonferroni post-hoc tests).