Figure 7.

The expression of TF and MMP9 are closely related to Trx1. LPS (0.3 μg/ml) was used to establish the endotoxemia model in HUVEC-C cells. LPS (1 μg/ml) was used to establish the endotoxemia model in THP-1 cells. H₂ (65% H₂) was used to treat both HUVEC-C and THP-1 cells. PX₁₂ (2 μM) was used in HUVEC-C cells as an inhibitor of Trx1. PX₁₂ (8 μM) was used in THP-1 cells as an inhibitor of Trx1. Effect of hydrogen on the expression of Trx1 in THP-1 (A) and HUVEC-C cells (B) were measured by western blot analysis and the densitometry values were normalized to β-actin at 6, 12, and 24 h (n = 3 in each group). Effect of hydrogen and PX₁₂ on the expression of TF in THP-1 (C) and HUVEC-C cells (D) at 12 h were measured by western blot analysis and the densitometry values were normalized to β-actin (n = 3 in each group). Effect of hydrogen and PX12 on the expression of MMP-9 in THP-1 (E) and HUVEC-C cells (F) were measured by gelatin zymography and the densitometry values were normalized at 12 h (n = 3 in each group). Significant differences were revealed following one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. LPS-treated group; ^$P < 0.05, ^$$$P < 0.001 vs. LPS + H₂ group; Bonferroni post-hoc tests).