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. 2021 Mar 9;12:650864. doi: 10.3389/fimmu.2021.650864

Figure 1.

Figure 1

α-TREM-1 prevents colitis and modulates the microbiota in mice. (A–F) Systemic administration of α-TREM-1 in DSS-treated mice. DSS was supplied in drinking water and IgG or α-TREM-1 was intraperitoneally injected (4 or 20 μg/mouse) into BALB/c mice. (G–N) The antibiotic (Abx) cocktail was supplied in drinking water 6 days before DSS administration (day 0) and IgG or α-TREM-1 (arrow: 20 μg/mouse) was intraperitoneally injected into BALB/c mice on day 0 (n = 8/groups). (A,G) Experimental design. (B,H) Body weight change. (C,I) Disease activity index. (D,J) Representative sections of periodic acid-Schiff stain. Scale bar, 100 μm. (E,K) Histological score. (F) PAS stain intensity. (L) Goblet cell score. (M) Bacterial load. Bacterial load in colon was assessed by 16S rRNA gene amplification. (N) Microbiota profiles (phylum level) in colonic tissues. Data are expressed as means ± S.E.M. (n = 4–8/groups). Statistical significance was assessed using one-way ANOVA followed by Tukey post-test. *P < 0.05, **P < 0.01, ***P < 0.005 (or vs. DSS+IgG). α-T, treated with α-TREM-1; IgG, treated with control antibody; Water, supplied with normal drinking water.