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. 2021 Mar 9;12:650864. doi: 10.3389/fimmu.2021.650864

Figure 2.

Figure 2

TLR4 signaling is required for the anticolitic effects of α-TREM-1. Wild-type (WT) and Tlr4-knockout (Tlr4−/−) BALB/c mice were subjected to a colitis and healing model with 3.5% DSS treatment for 7 days and normal drinking water for 2 days (n = 7/groups). The arrow indicates the point at which IgG- or α-TREM-1 (α-T) was administered (20 μg/mouse). (A) Experimental design. (B) Body weight change. (C) Disease activity index. (D) Representative sections of PAS stain. Scale bar, 100 μm. (E) Histological score. (F) Goblet cell score. (G) Total number of bacterial OTUs (left), richness predicted by the Chao1 index (middle), and diversity by the Shannon index (right) in the colon. (H) Microbiota profiles in the colon at the phylum level. Statistical significance was assessed using one-way ANOVA followed by Tukey post-test. *P < 0.05, **P < 0.01, ***P < 0.005. PBS administered phosphate-buffered saline; Water, supplied with normal drinking water.