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. 2021 Mar 9;12:650864. doi: 10.3389/fimmu.2021.650864

Figure 4.

Figure 4

Neutrophils and macrophages mediate α-TREM-1-induced anti-colitic effects. BALB/c mice were intraperitoneally given an anti-Gr1 antibody (Ly6G) twice to deplete neutrophils or clodronate (Clo) twice to deplete macrophages. Both these groups were then injected once with IgG or α-TREM-1 (20 μg/mouse) at day 2. After DSS treatment for 8 days, DSS was exchanged for drinking water for 2 days (n = 4–6/groups). (A) Experimental design. (B) Disease activity index. (C) Representative sections of a periodic acid-Schiff stain. Scale bar, 100 μm. (D) Histological score. (E) Densitometry analysis of periodic acid-Schiff stain. (F) Flow cytometric analysis of macrophages and CD177+ neutrophils in lamina propria mononuclear cells. (G) qRT-PCR analysis of Inos expression profiles in colons. Each data represents the mean of duplicate real-time RT-PCR (n = 3–4). Data are expressed as means ± S.E.M. Statistical significance was assessed using one-way ANOVA followed by Tukey post-test (B,G) or Student t-test (D,E). *P < 0.05, **P < 0.01, ***P < 0.005. α-T, treated with α-TREM-1; IgG, treated with control antibody; Water, supplied with normal drinking water.