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. 2021 Mar 9;11:614035. doi: 10.3389/fonc.2021.614035

Figure 1.

Figure 1

miR-4461 knockdown inhibits ovarian cancer cells proliferation. (A) Real-time PCR analysis miR-4461 levels in OC tissues and normal tissues. (B) The knockdown and overexpression effect of miR-4461 in HO8910 and A2780 cells was detected by real-time PCR analysis. (C) Cell proliferation in miR-4461 sponge or miR-4461 mimic and their control cells was quantified by using CCK-8 assays. Cisplatin (1 μg/ml) used as a positive control. (D) Colony formation assays of HO8910 miR-4461 sponge or A2780 miR-4461 sponge and their control cells. Scale bar = 100 μm. (E) Representative images of EdU staining of proliferating miR-4461 sponge or miR-4461 mimic and their control cells. EdU+ cells were stained with red immunofluorescence. The nuclei were counterstained with DAPI (blue). Cisplatin (1 μg/ml) used as a positive control. Scale bar = 50 μm. (F) Cell cycle in HO8910 miR-4461 sponge or miR-4461 mimic and their control cells was assessed by flow cytometry. Cisplatin (1 μg/ml) used as a positive control. (G) Cell cycle in A2780 miR-4461 sponge or miR-4461 mimic and their control cells was assessed by flow cytometry. Cisplatin (1 μg/ml) used as a positive control. *means p < 0.05, statistical difference.