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. 2021 Mar 9;11:614035. doi: 10.3389/fonc.2021.614035

Figure 3.

Figure 3

PTEN is a direct target of miR-4461 in ovarian cancer cells. (A) A potential target site for miR-4461 in the 3′-UTR of human PTEN mRNA, as predicted by the program Targetscan and miRBase. To disrupt the interaction between miR-4461 and PTEN mRNA, the target site was mutated. (B) Luciferase reporter assays performed in HO8910 miR-4461 sponge or A2780 miR-4461 sponge and their control cells transfected with wild-type or mutant PTEN 3′-UTR constructs. (C) Luciferase reporter assays performed in HO8910 miR-4461 mimic or A2780 miR-4461 mimic and their control cells transfected with wild-type or mutant PTEN 3′-UTR constructs. (D) The mRNA expression of PTEN was determined in HO8910 miR-4461 sponge or A2780 miR-4461 sponge and their control cells by real-time PCR. (E) The mRNA expression of PTEN was determined in HO8910 miR-4461 mimic or A2780 miR-4461 mimic and their control cells by real-time PCR. (F) The protein expression of PTEN was determined in HO8910 miR-4461 sponge or A2780 miR-4461 sponge and their control cells by western blot. (G) The protein expression of PTEN was determined in HO8910 miR-4461 mimic or A2780 miR-4461 mimic and their control cells by western blot. (H) Significant correlation was observed between miR-4461 and PTEN expression in human ovarian cancer tissues (n = 40). *means p < 0.05, statistical difference.