Figure 2.

Oncolytic potential of OAd-NIS vectors

(A) Crystal violet staining of pancreatic cancer and Cox2^– BT474 cells. The human A549 lung carcinoma cell line was used as standard CAR-, Ad3-receptor-, and Cox2⁺ control. Wild-type Ad5 virus (Ad5WT) was used as a positive control to evaluate the cytolytic potential of recombinant vectors. The oncolytic effect of 5/3 fiber-modified, Cox2-controlled vectors was comparable to that of Ad5Wt. Staining of Cox2^– BT474 cells shows susceptibility to WT viruses but resistance to Cox2-controlled viruses. (B) Cell viability MTS assay of A549 and Panc1 cells. Error bars represent standard deviation. All viruses were able to eliminate all tumor cells; however, ADP(–) viruses killed cells more slowly than ADP(+) viruses.